The HSC70/HSP70 monoclonal antibody (N27F3-4) from MyBioSource.com is a Mouse Monoclonal antibody to HSPA1A, and HSPA1B. This antibody recognizes Human, Mouse, Rat, Bovine, Canine, Chicken/Bird, Guinea Pig, Hamster, Non-Human Primate, Plant, Porcine, Rabbit, Sheep, Xenopus/Amphibian, Zebrafish/Fish, and Other Mammalian antigen. The HSC70/HSP70 monoclonal antibody (N27F3-4) has been validated for the following applications: Flow Cytometry, Immunohistochemistry, Immunohistochemistry - fixed, Immunoprecipitation, and Western Blot.
Description
The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway